2010•Zhongguo shouyi xuebaoRequires access
Serotyping of Guangxi isolates of infectious bronchitis virus
Xiuying Wang, Ping Wei, Chen QiuYing, Meng Li, Meilan Mo, Shengbin Zhang, Wei FeiNi
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Abstract
Monovalent antisera against seven field isolates and three commonly used vaccine strains of infectious bronchitis virus (IBV) were prepared in rabbits and were used in the virus neutralization test against the heterogeneous and homogeneous viruses on tracheal organ cultures (TOC) of chicken embryo.Relatedness values of each virus calculated from cross-neutralization antibody titer showed that 6 serotypes were distinguished among the 10 viruses tested.Phylogenetic analysis of the different serotypes of viruses also indicated that there was a divergence among the field isolates and between the isolates and the vaccine strains,belonging to different serotypes.The data of the study provides a reliable basis for catching up the emerged antigenic variation of the field viruses and developing more effective vaccine for the disease control in Guangxi.The relationship between genotyping and serotyping was also discussed.