Experimental Research of the Impacts of Shenxian Mixture on the Bone Marrow Suppression and Thrombopoietin in Cyclophosphamide Model Mice

Objective To study the impacts of shenxian mixture on the bone marrow suppression and thrombopoietin(TPO)in cyclophosphamide induced model mice.Methods Kunming mice were randomized into six groups,8 mice in each one,named a blank group,a model group,a positive control group,a low-dose group,a middle-dose group and a high-dose group of shenxian mixture.In each shenxian mixture group,the drug lavage was done for 5 days.Since the 6th day,the intraperitoneal injection of cyclophosphamide 100 mg·kg-1 was applied in each group except the blank group,continuously for 3 days for modeling.In the positive control group,the subcutaneous injection of recombinant interleukin-11(rh LI-11),200 μg·kg-1 was given,continuously for 6 days.The drug lavage was continued in each shenxian mixture group for the other 6 days.The white blood cell(WBC)and platelet(PLT)counts were detected regularly for the mice in each group.On the 12th day of the experiment,the bone marrow megakaryocyte count and TPO concentration in the serum and bone marrow supernatant were detected.Results WBC,PLT and megakaryocyte counts in each shenxian mixture group were higher significantly than those in the model group(P0.05 or P0.01).The megakaryocyte count in the positive control group was higher significantly than that in the model group(P0.01).TPO concentration in the serum and bone marrow supernatant in each shenxian mixture group and the positive control group was higher significantly than that in the model group(P0.01).The positive relationship was presented between TPO concentration and the dose of shenxian mixture,indicating the significant dose-effect relationship.Conclusion Shenxian mixture improves TPO level in bone marrow and blood so that it promotes the formation of megakaryocytes and platelets,prevents and treats bone marrow suppression.