Effects of high mobility group box protein-1 on the secreting of insulin-like growth factor-1 and apoptosis of the mouse bone marrow-derived mesenchymal stem cells

Objective To investigate effects of High mobility group box protein-1 (HMGB1) on the secreting of insulin-like growth factor-1 (IGF-1) and apoptosis of the mouse bone marrow-derived mesenchymal stem cells (BMSCs). Methods To isolate and culture BMSCs of Kunming mouse in vitro, detect the expression rate of the 3rd generation BMSCs surface antigens CD34, CD44 by flow cytometry. After intervention with different concentrations of HMGB1 (0, 12.5, 25, 50, 100 ng/mL), the IGF-1 concentration in the supernatant fluids was measured by enzyme-linked immunosorbent assay (ELISA), apoptosis rate of BMSCs was determind by flow cytometry. Results The expression rate of surface antigen CD44 of the 3rd generation attached cell cultured in vitro was 96.7%, the expression rate of CD34 was 1.1%. After 24 h, the concentrations of IGF-1 in supernatant were significantly different in 25,50,100 ng/mL groups and 0 ng/mL group (P 0.05). After 48 h, the differences of the concentrations of IGF-1 in supernatant were significant in 12.5, 25, 50, 100 ng/mL groups and 0 ng/mL group (P 0.05). The apoptosis rates of BMSCs in each group after 72 h in 50, 100 ng/mL groups and 0 ng/mL group were obviously significant (P 0.05). Conclusions Appropriate concentration of HMGB1 (25 ng/mL) can promote BMSCs secreting IGF-1 and a larger concentration of HMGB1 (≥ 50 ng/mL) could significantly induce apoptosis in BMSCs.