The toxicity of β-sitosterol on the vitality of hippocampal neuron cell in original generation rats

Objective: To study sitosterol toxic effects on normal and epileptic discharge hippocampal neurons.Methods: Wistar rats neonatal in 24h were used as cell source,with Neurobasal culture medium cultured for 9 days,without Mg2+ treated for 3 h,to induce epileptic discharge,and sitosterol alcohol(concentration) was added to react for 24h.MTT assay was used to check the effect of beta-sitosterol on hippocampal neurons non-toxic and primary rat hippocampal neurons in epileptic cells for the best time.Results:We succeeded in isolating the newborn rat hippocampal nerve cells,and the non-toxic concentration of sitosterol was 20μg/mL,and the best time was 24h.Conclusion: Sitosterol concentration of 20μmol/L,for 24 hours,are not toxic for hippocampal neurons,which can be used for the treatment of epilepsy.