Essential developmental biology: a practical approach.

Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes and Philip Ingham nematode embryos, Lois G. Edgar and William B. Wood embryos and larvae of invertebrate deuterostomes, Nicholas D. Holland and Linda Z. Holland zebrafish embryos, Rachel M. Warga Xenopus, Ariel Ruis I. Altaba avian embryos, Claudio D. Stern post-implantation of mammalian embryos, Gillian M. Morriss-Kay simple tips for photomicrography of embryos, Claudio D. Stern. Part 2 Experimental embryology: fate maps and cell lineage analysis, Ariel Ruiz I. Altaba et al micro-injection into drosophila embryos, Philip Ingham and Zandy Forbes cell transplantation in xenopus, Ariel Ruiz I. Altaba transplantation in avian embryos, Claudio D. Stern chick limb buds, Cheryll tickle nuclear transplantation, John B. Gordon laser ablation of cells, Judith Eisen et al. Part 3 Cellular techniques: studying cell movements in vivo by time-lapse video microscopy, Peter V. Thorogood and Dee Amanze induction assays in Xenopus, Ariel Ruiz I. Altaba culture of avian neural crest cells, Elisabeth Dupin and Nicole M. Le Douarin embryonic stem cells and gene targeting, Elizabeth J. Robertson and Gail R. Martin introduction of genes using retroviral vectors, Jack Price. Part 4 Molecular techniques: immonocytochemistry of embryonic material, Claudio D. Stern RNAase protection assays, Ariel Ruiz I. Altaba cDNA library construction, Akira Kakizuka et al analysis of gene expression by reverse transcription polymerase chain reaction, Peter Koopman cloning genes using the polymerase chain reaction, Peter W.H. Holland in situ hybridization, David G. Wilkinson. Appendices: stage tables suppliers of specialist items.