2006•Chinese Journal of Otorhinolaryngology-skull Base SurgeryRequires access
Establishment of a human laryngeal squamous cell carcinoma cell line expressing HPV16-E6/E7 gene
Jinrong Li
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Abstract
Objective To establish a human laryngeal squamous cell carcinoma cell line expressing human papillomavirus(HPV16) E6/E7 oncogene.Methods Cationic phosphonolipid was used to transfect pLXSN16E6E7 into a HPV16-negative laryngeal squamous cell carcinoma cell line in passage number 20,Lscc-02.The expression of HPV16-E6/E7 in Lscc-02 cell line was detected by PCR and Western blot.Results HPV16-E6/E7 DNA was found in the transfected cell line by PCR and E6/E7 protein could be detected by Western blot in either passage number 22 or passage number 35,which showed that HPV16-E6/E7 was expressed stably in the transfected cell line.Conclusion A human laryngeal squamous cell carcinoma cell line expressing HPV16-E6/E7 has been established successfully.The newly established cell line may serve as a useful model for studying the role of HPV16-E6/E7 in laryngeal carcinoma cells and gene therapy of laryngeal carcinoma.