Effects of target blocking COX-2 on the growth of laryngeal cancer

Objective To explore the effect of COX-2 inhibitor(Celecoxib) on laryngeal cancer cell lines Hep-2.Methods Laryngeal cancer cell lines Hep-2 was treated with Celecoxib at various concentrations.Cell growth inhibition rate was measured by MTT.After Hep-2 cells were treated with Celecoxib at the concentration of 15 μmol/L,the cell cycle and apoptosis of the cells were measured by flow cytometric analysis.The expression of relative proteins was also examined using western blot.Results ① The cell growth inhibition rate of Celecoxib-treated group was much higher than that of the control,and the inhibition was concentration-dependent in a certain range.② The G1 ratio and apoptotic rate of Celecoxib-treated group were significantly higher than those of the control group respectively(P0.05).③ Compared with the control group,the expression of p-EGFR and COX-2 in Celecoxib-treated group decreased significantly(P0.05).Conclusion Celecoxib can inhibit the growth and induce apoptosis of Hep-2 cell lines via significant induction of Hep-2 cells G1.Meanwhile,Celecoxib can reduce phosphorylation of EGFR and expression of COX-2.Therefore,Celecoxib,as a COX-2 inhibitor,may be a new agent for targeting therapy of laryngeal cancer.