EFFECTS OF HUAYU RUFEI DECOCTION ON TGF-β_1,COLLAGEN I AND III EXPRESSIONS IN LUNG TISSUE OF RATS WITH PULMONARY FIBROSIS

Objective:To discuss the intervention effect and the machanism of huayu rufei decoction on rats' pulmonary fibrosis induced by bleomycin A5.Methods: 60 male Wistar rats were divided into normal,model,high and low dose decoction groups at random,each group included 15 rats.Rats' pulmonary fibrosis models were made by single intracheal instillation of bleomycin(5mg/kg), each group rats were given corresponding drugs respectively,5 rats were killed in each group after modeling 7,14 and 28 days,the expressions of TGF-β1,collagen Ⅰ and Ⅲ in lungs of each group rats were determined by immunohistochemistry method.Results: To compare with the model group,the severity of rats' lung inflammation and fibrosis were lightened in the two Chinese herbs groups,the expressions of TGF-β1,collagen Ⅰ and Ⅲ in lungs were reduced more obviuosly.Conclision: Huayu rufei decoction can relieve pulmonary fibrosis,inhibiting the expressions of TGF-β1,collagen Ⅰ and Ⅲ in lungs maybe one of its mechanism.