Apoptosis of Hela cells induce by hydroxyapatite nanoparticles

Objective: To study the effects of different of concertrations of hydroxyapatite nanoparticles on human Hela cell line.Method:The Hela cell was treated with HAP nanopartricles at various concentrations for different duration . Growth suppression was evaluated by MTT method;apoptosis-related alterations in morphology were determined by transmission electron microscopy(TEM).The apoptotic was detected under TdT-mediated dUTP Nick-epd Labeling(TUNEL).Results:HAP nanoparticles inhibited the growth of Hela cells in a dose and time dependent manner ,with IC50 values of 211.8μg/ml after 48h atratment.the cells treated with HAP nanopariticles exhibited characteristics of apoptosis under TEM. Conclusion: HAP nanoparticles not only inhibit the proliferation but also induce apoptosis of Hela cell .HAP nanoparticles provides a basis for treatment of tumor.