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A preliminary study on the inactivation in vitro to tobacco mosaic virus (TMV) by anti viral agent VA from plants

Hong Li

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Abstract

VA , a anti viral agent from plants, could inactivate TMV in vitro , when it was mixed with TMV and inoculated by means of half leaf. The inhibition rate of necrotic local lesions was 80.0%. Ribonucleic acid extracted from TMV was mixed with VA in proportion, then inoculated onto leaves of tobacco. The experimental results showed that the inhibition rates of necrotic local lesions were from 43.8% to 77.9%. While RNase was added to the mixture of TMV and VA, the infection rate of TMV was 78 6%, decreased by 21 4% in contrast with the check. Meanwhile, it was showed that VA broke or made TMV particles become non equipollent thickness or curve slightly through transmission electron microscope (TEM).

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What this paper is about

VA , a anti viral agent from plants, could inactivate TMV in vitro , when it was mixed with TMV and inoculated by means of half leaf. The inhibition rate of necrotic local lesions was 80.0%. Ribonucleic acid extracted from TMV was mixed with VA in proportion, then inoculated onto leaves of tobacco. The experimental results showed that the inhibition rates of necrotic local lesions were from 43.8% to 77.9%. While RNase was added to the mixture of TMV and VA, the infection rate of TMV was 78 6%, decreased by 21 4% in contrast with the check. Meanwhile, it was showed that VA broke or made TMV particles become non equipollent thickness or curve slightly through transmission electron microscope (TEM).

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Available abstract

VA , a anti viral agent from plants, could inactivate TMV in vitro , when it was mixed with TMV and inoculated by means of half leaf. The inhibition rate of necrotic local lesions was 80.0%. Ribonucleic acid extracted from TMV was mixed with VA in proportion, then inoculated onto leaves of tobacco. The experimental results showed that the inhibition rates of necrotic local lesions were from 43.8% to 77.9%. While RNase was added to the mixture of TMV and VA, the infection rate of TMV was 78 6%, decreased by 21 4% in contrast with the check. Meanwhile, it was showed that VA broke or made TMV particles become non equipollent thickness or curve slightly through transmission electron microscope (TEM).

Key concepts: Tobacco mosaic virus, In vitro, Inoculation, Virology, RNase P, Tobacco leaf, Virus, Chemistry

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