Protective Action on Transplants BMSCs by Chinese Traditional Medicine Xinfukang after Rats Acute Myocardial Infarction

Objective To discuss the protective action on transplanted BMSCs by Chinese traditional medicine XinFuKang(XFK) after rats acute myocardial infarction.Methods BMSCs were cultured and purified by gradient centrifugation-adhesive method.The rats with acute myocardial infarction were built by coronary artery ligation.Before transplation,BMSCs were signed by Brdu,and after the OPS of coronary artery ligation,the therapy methode was XFK combining BMSCs transplantation.After 2 weeks,we measured serum VEGF by method of ELISA,the albumen VEGF,cell amount of Brdu(+) and neonatal vessels of CD34+ around the infarct area by method of IHC.Results The method of XFK combining BMSCs transplantation increased livability of transplanted BMSCs and serum VEGF(compared with group of BMSCs tranplation,P0.05),enhanced infarct area albumen VEGF and neonatal vessels.Conclusion Compared with simply BMSCs transplantation,XFK hase better protection on the transplanted BMSCs.The mechanism maybe XFK can promote the vessels regeneration of infarct area.