PROTEOMICS ANALYSIS FOR THE MOLECULAR MECHANISM OF TAURINE AGAINST IMPAIRED MEMORY INDUCED BY ACUTE MANGANESE ADMINISTRATION

Objective To investigate the molecular mechanism of taurine against impaired study and memory capacity induced by acute MnCl2 administration.Method Sixty SPF male rats were randomly divided into 3 groups.Control group was peritoneally injected saline everyday for 4 w.MnCl2-treated group was injected i.p.15 mg/(kg.d) MnCl2 for 4 w,Taurine-prevented group was injected i.p.both MnCl2 and 200 mg/kg.d taurine together for 4 w.Rats were trained to proceed Morris water maze to determine study and memory capacity.Hippocampus was separated to prepare for 2-DE.The variant spots were collected and identified by MALDI-TOF-MS and Mascot software.Results The average escape latency of rats in MnCl2-treated group was(39.8±2.3 s),markedly longer than those in control(29.5±2.5s),and taurine-prevented(29.4±2.3s) group(P0.05).But the probing times were not significantly different among different groups.4 of 10 common variant spots from 3 groups were identified.There were NADH dehydrogenase(ubiquinone) Fe-S protein 1,galactokinase I,whaq protein,beta-centractin.Conclusion Taurine improves impaired study and memory capacity induced by MnCl2.Proteomics analysis reveals that 4 down-regulated spots may be responsible for taurine action against MnCl2 neurotoxicity.[ACTA NUTRIMENTA SINICA,2012,34(6):553-557]