Cloning and Sequence Analysis of the Late Embryogenesis Abundant Related Protein Gene from Hevea brasiliensis

Using the primers designed from the known EST from a suppression subtractive hybridization-cDNA library,the full-length cDNA sequence of HbLEA14 was cloned from Hevea brasiliensis latex by 3'-RACE technique.The opening reading frame(ORF)of HbLEA14 was 456 bp in length,encoding a 151-amino-acid polypeptide with a predicted molecular weight of 16.58 ku and isoelectric point of 5.10.Cluster analysis revealed that HbLEA14 was an atypical LEA2,and showed 88% identity with Ricinus communis RcLEA14,80% to Euphorbia esula EeLEA14,75% to Gossypium hirsutum GhLEA14,73% to Glycine max GmLEA14,65% to Arabidopsis thaliana AtLEA14 and 61% to Solanum lycopersicum SlER5,respectively.In addition,HbLEA14 exhibited higher transcriptional expression level in the latex of tapping panel dryness trees than in healthy trees.This paper will benefit to further analyze the functions of HbLEA14.