ScholarLens Back to search
2015•Chinese Journal of Modern Drug ApplicationRequires access

Simultaneous determination of serum concentration of 4 antiepileptic drugs by RP-HPLC

Hu We

Open publisher page 0 citations

Abstract

Objective To establish a simultaneous determination method by high performance liquid chromatography for serum concentration of lamotrigine(LTG), phenobarbital(PB), phenytoin(PHT), and carbamazepine(CBZ). Methods After protein precipitation by methyl alcohol, the serum samples were taken for determination with chromatographic column of Waters Symmetry C18 column(250 mm×4.6 mm, 5 μm), methyl alcohol-water(55∶45) as moving phase and detection wavelength as 235 nm. Results The linear ranges of LTG, PB, PHT, and CBZ were respectively 1.56~50, 3.13~100, 2.19~70, and 1.09~35 μg/ml. The average recovery rate was 98%. Conclusion This method is easy operational with precise effect. It is suitable for monitoring of clinical treatment drugs.

About this research paper

What this paper is about

Objective To establish a simultaneous determination method by high performance liquid chromatography for serum concentration of lamotrigine(LTG), phenobarbital(PB), phenytoin(PHT), and carbamazepine(CBZ). Methods After protein precipitation by methyl alcohol, the serum samples were taken for determination with chromatographic column of Waters Symmetry C18 column(250 mm×4.6 mm, 5 μm), methyl alcohol-water(55∶45) as moving phase and detection wavelength as 235 nm. Results The linear ranges of LTG, PB, PHT, and CBZ were respectively 1.56~50, 3.13~100, 2.19~70, and 1.09~35 μg/ml. The average recovery rate was 98%. Conclusion This method is easy operational with precise effect. It is suitable for monitoring of clinical treatment drugs.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish a simultaneous determination method by high performance liquid chromatography for serum concentration of lamotrigine(LTG), phenobarbital(PB), phenytoin(PHT), and carbamazepine(CBZ). Methods After protein precipitation by methyl alcohol, the serum samples were taken for determination with chromatographic column of Waters Symmetry C18 column(250 mm×4.6 mm, 5 μm), methyl alcohol-water(55∶45) as moving phase and detection wavelength as 235 nm. Results The linear ranges of LTG, PB, PHT, and CBZ were respectively 1.56~50, 3.13~100, 2.19~70, and 1.09~35 μg/ml. The average recovery rate was 98%. Conclusion This method is easy operational with precise effect. It is suitable for monitoring of clinical treatment drugs.

Key concepts: Carbamazepine, Chromatography, Chemistry, Phenytoin, Protein precipitation, Lamotrigine, Phenobarbital, High-performance liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Simultaneous determination of serum concentration of 4 antiepileptic drugs by RP-HPLC — Research Paper | ScholarLens