Producing Laccase-characteristics of Marasmius tricolor Isolated from the Soil of Alpine Meadow

A fungus strain belonging to Marasmius tricolor has been isolated and selected from the soil of alpine meadow located in eastern Qilian Mountains.To full understand the characteristics of the fungus producing the laccase,three substrates(i.e.,guaiacol,caechol,and o-benzyl aniline) were used to induce the activity of laccase,using substrate spectrophotometry and liquid rotating methods.The temporal order and sizes that the oxidation zones occurred on guaiacol-PDA culture media were analyzed to investigate the relative rates that the fungus produced laccase.The results showed that Marasmius tricolor produced oxidation zones cultured on the media that contained guaiacol,caechol,and o-benzyl aniline,respectively,but the colors and diameter sizes of the oxidation zones and fungus community diameters differed from different media.The diameters of the oxidation zones and fungus community were the greatest when the fungus was cultured on the medium that contained guaiacoI.The fungus showed the greatest activity producing laccase.Guaiacol might induce its hypha to secrete laccases at the early stage of its growth.Laccase activity was the highest when guaiacol was substrate in the reaction system.Laccase produced by the fungus showed the greatest activity under the condition of 30~C and pH 4.5.The enzyme also showed higher activity under the condition of 0~45℃,pH 4.0~6.5.