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Effects of nutrition and environment on oil biodegradation by bacterial community

Jiang Rui-bo

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Abstract

Oil degrading bacteria were isolated and bacterial community C9 with oil degrading ability was constructed.KNO3,K2HPO4,pH 7～10 and 150～200 r/min were determined as the best N source,P source,initial pH values,and rotating speed respectively by sole factor experiment for C9.The orthogonal experiment results showed that the most suitable proportion of strains SL51∶SL-84∶SL-133∶SL-163 was 1∶1∶20∶1,and optimum oil degradation condition was KNO3 3 g·L-1,K2HPO4 1 g·L-1,and pH 8 respectively,at the same time initial inoculation volume was 6.9%,oil concentrate was 0.25%.Gas chromatography analysis proved that C9 could degrade saturated hydrocarbon and aromatic hydrocarbon more effectively in the optimum conditions.

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Oil degrading bacteria were isolated and bacterial community C9 with oil degrading ability was constructed.KNO3,K2HPO4,pH 7～10 and 150～200 r/min were determined as the best N source,P source,initial pH values,and rotating speed respectively by sole factor experiment for C9.The orthogonal experiment results showed that the most suitable proportion of strains SL51∶SL-84∶SL-133∶SL-163 was 1∶1∶20∶1,and optimum oil degradation condition was KNO3 3 g·L-1,K2HPO4 1 g·L-1,and pH 8 respectively,at the same time initial inoculation volume was 6.9%,oil concentrate was 0.25%.Gas chromatography analysis proved that C9 could degrade saturated hydrocarbon and aromatic hydrocarbon more effectively in the optimum conditions.

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Available abstract

Oil degrading bacteria were isolated and bacterial community C9 with oil degrading ability was constructed.KNO3,K2HPO4,pH 7～10 and 150～200 r/min were determined as the best N source,P source,initial pH values,and rotating speed respectively by sole factor experiment for C9.The orthogonal experiment results showed that the most suitable proportion of strains SL51∶SL-84∶SL-133∶SL-163 was 1∶1∶20∶1,and optimum oil degradation condition was KNO3 3 g·L-1,K2HPO4 1 g·L-1,and pH 8 respectively,at the same time initial inoculation volume was 6.9%,oil concentrate was 0.25%.Gas chromatography analysis proved that C9 could degrade saturated hydrocarbon and aromatic hydrocarbon more effectively in the optimum conditions.

Key concepts: Biodegradation, Hydrocarbon, Bacteria, Degradation (telecommunications), Volume (thermodynamics), Chemistry, Environmental science, Food science

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