2007•Chinese Journal of Gastroenterology and HepatologyRequires access
Effect of leptin on proliferative activity of human hepatocellular carcinoma cells Huh 7
Deying Tian
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Abstract
Objective To explore the effect of leptin on proliferative activity of human hepatocellular carcinoma cells Huh 7 and its molecular mechanism. Methods Huh 7 cells were treated with different concentrations of leptin. Cell counting, MTT assay, flow cytometry and Western blot were used to observe the changes of cell growth curve, cell proliferation, cell cycle and Cyclin D1 protein expression, respectively. Results Leptin could significantly improve the proliferation rate of Huh 7 cells. The effect is in dose-and time-depended by MTT method. In the flow cytometry analysis, it was found that leptin could promote Huh 7 cells to enter S phase from G0/G1 phase and the apoptosis rates were also significantly decreased. The expression of Cyclin D1 was increased in Huh cells treated with leptin. Conclusion Leptin could promote the proliferation of Huh 7 cells, and its mechanism may be that it can increase the expression of Cyclin D1.