Construction and Identification of Recombinant Vectors Expressing Antisense RNA to CCR_5

objective To construct recombinant vector expressing antisense RNA to CCR5-an entry co - receptor for HIV- 1 infection, which will be used to block HIV- 1 infection. Methods The DNA fregment target to the translation initiation site of CCR5 mRNA obtained by RT - PCR from peripheral blood mononuclear cells(PBMCs)was cloned into retro-viral vector pLXSN.The resultant vectors were identified by PCR, restriction endonucleases analysing and DNA sequencing, then transfected into packaging cell(PA317)with lipofectAMINE and detected by genomic PCR. Results The recombinant vector expressing antisense/sense RNA to CCR5 was constructed. The vector had incorporated into the genome of transfected PA317cells. Conclusion The experiments make a great foundation for further studying the effect of antisense RNA to CCR5 on HIV- 1 infection.