The Effect of Heat Shock Protein 70 on Myocardial Interstitium of Isolated Rat Hearts

Objective To investigate the protective effects of heat shock protein 70(HSP_(70)) on myocardial interstitium of isolated rat heart.Methods 16 Wistar rats were randomly divided into 2 groups.In controll group(C,n=8),distilled water was injected intraperitioneally and 24 hours later isolated hearts were perfused by Langendorff model and stored in 4℃for 3 hours with Histidine-trytophan-ketoglurate(HTK) solutions,and then isolated hearts Langendorff model was perfused for 2 hours.In experimental group(E,n=8),norepinephrine was injected intraperitioneally and 24 hours later isolated hearts were perfused by Langendorff model and stored in 4℃for 3 hours with HTK solution,and then isolated hearts Langendorff model was perfused for 2 hours.HSP_(70) content,the recovery of hemodynamics,hydroxyproline(HP) and endothelin(ET) content,and ultrastructure were tested.Results There was significant increase in HSP_(70),hemodynamics recovery and HP content in group E than group C,and significant decrease in ET in group E than in group C.Ultrastructure injury in group E is less than that in group C.Conclusion This study demonstrated that HSP_(70) has significant protective effect on myocardial interstitium of isolated rat heart.