ENHANCEMENT OF THE GRAFTING EFFICIENCY BY THE NEW METHOD OF FETAL LIVER-BONE MARROW SCHEDULED TRANSPLANTATION

To enhance the grafting efficiency of bone marrow transplantation. Lethally Irradiated recipient Kunming mice were transplantation with fetal liver-bone marrow scheduled transplantation. (FL-BMST) The numbers of WBC, nucleated cells were near to normal Ievel 17d after irradiation in FL-BMST group transplantation with 1 x 10~6 bone marrow cells. the indexes of CFU-E, CFU - GM, CFU-F, CFU-S, were returned to normal; the degree of GVHD in the FL-BMST group was slighter than that in sing bone marrow transplantation group: and the survival rate of mice was 60%, which was significantly higher than that of routine single bone marrow transplantation group. Niches vacated each time could be fully used and be improved, be increased by fetal liver-bone marrow scheduled transplantation, so the homing of stem cells was increased, and the number of transplanted bone marrow cells could be decreased. So this new method was a better method than routine bone singe marrow transplantation.