Implication of transforming growth factor-β1 expression in the repaired bile duct defect with free parietal peritoneal graft

Objective:To observe the expression of transforming growth factor-β1(TGF-β1) expression in the repaired bile duct defect with free parietal peritoneal graft and explore the feasibility of using autogenous free peritoneal graft for the repair of bile duct defect, and desire to find ideal material for the clinical management of bile duct injury.Methods:(1)24 local healthy mongrel dogs underwent right rectus abdominis laparotomy and had their bile duct resected to establish bile duct injury models. The parietal peritoneal flap was harvested near the abdominal incision with the similar dimension of bile duct defect, and was sutured to the edges of the excised bile duct with a coverage of omentum outside. (2)Laparotomy was performed tissues of common bile duct, especially the repaired segment, was harvested, and the expression of TGF-β1 was studied by using immunohistochemical SP staining method.Results:TGF-β1 was expressed strongly before 12 weeks, but after 28 weeks, TGF-β1 were expressed weakly, which had no significant difference from preoperation(P0.05).Conclusion:Free autogenous peritoneum can be used for regeneration of bile duct tissue in a canine model because of the low expression of TGF-β1,which indicates impossibility of biliary stricture.