AC133 Expression in Brain of Ischemia-Reperfused Rat

Aim:To determine whether or not there was AC133 expression in brain of focal brain ischemia/reperfused rat.Methods:Right middle cerebral artery suture occlusion model was used.42 adult SD male rats were randomly divided into 3 groups:control,sham-control and cerebral ischemia/reperfusion model which included 2d,3d,4d,7d reperfusion groups.After right middle cerebral artery occluded,the rats were suffered from ischemia for 2 hours,then reperfusion was allowed for different time as mentioned above before decapitation.Cerebral AC133 antigen expressions in various point were tested by immunohistochemistry,and AC133 mRNA expressions in 3d,7d reperfusion groups were detected by RT-PCR.Results:AC133 antigen expression was only observed in certain endothelial cells of around infarct core in 4d group.AC133 mRNA expression was upregulated in the groups of ischemia-reperfusion compared with normal,and increased levels in ispilateral hemisphere of infarction were higher than that in contralateral hemisphere.Conclusion:AC133 may take part in vessel function in rat MCAO models after focal cerebral ischemia/reperfusion.