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Experimental study on the effects of Resveratrol on cell cycle of HepG2 cells

Meizhi Wang

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Abstract

Objective:To discuss the effects of Resveratrol(Res) on the growth and cell cycle of human hepatoblastoma-derived HepG2 cell line.Methods: HepG2 cells were exposed to Res at the different concentrations(0,25,50 and 100 μmol/L)for 24h,48h and 72h.Morphologic changes of cells were detected under the phasecontrast microscope.The cell cycle of HepG2 cell treated with Res were analyzed by flowcytometry.Cyclin dependent kinase 2(CDK2) and Cyclin E expression level were tested by flowcytometry.Results: Res could significantly inhibit proliferation of HepG2 and cause the increase of cells in the S phase,G2/M and a corresponding decrease of cells in the G0/G1 phase.Expression level of CDK2 and Cyclin E were also increased in treated cells with a dose-dependent manner.Conclusions: Res will inhibit the proliferation of HepG2 in vitro,by inducing cell blockage at G0/G1 stage,which maybe resulted from the inactivation of CDK2 and Cyclin E.

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Objective:To discuss the effects of Resveratrol(Res) on the growth and cell cycle of human hepatoblastoma-derived HepG2 cell line.Methods: HepG2 cells were exposed to Res at the different concentrations(0,25,50 and 100 μmol/L)for 24h,48h and 72h.Morphologic changes of cells were detected under the phasecontrast microscope.The cell cycle of HepG2 cell treated with Res were analyzed by flowcytometry.Cyclin dependent kinase 2(CDK2) and Cyclin E expression level were tested by flowcytometry.Results: Res could significantly inhibit proliferation of HepG2 and cause the increase of cells in the S phase,G2/M and a corresponding decrease of cells in the G0/G1 phase.Expression level of CDK2 and Cyclin E were also increased in treated cells with a dose-dependent manner.Conclusions: Res will inhibit the proliferation of HepG2 in vitro,by inducing cell blockage at G0/G1 stage,which maybe resulted from the inactivation of CDK2 and Cyclin E.

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Available abstract

Objective:To discuss the effects of Resveratrol(Res) on the growth and cell cycle of human hepatoblastoma-derived HepG2 cell line.Methods: HepG2 cells were exposed to Res at the different concentrations(0,25,50 and 100 μmol/L)for 24h,48h and 72h.Morphologic changes of cells were detected under the phasecontrast microscope.The cell cycle of HepG2 cell treated with Res were analyzed by flowcytometry.Cyclin dependent kinase 2(CDK2) and Cyclin E expression level were tested by flowcytometry.Results: Res could significantly inhibit proliferation of HepG2 and cause the increase of cells in the S phase,G2/M and a corresponding decrease of cells in the G0/G1 phase.Expression level of CDK2 and Cyclin E were also increased in treated cells with a dose-dependent manner.Conclusions: Res will inhibit the proliferation of HepG2 in vitro,by inducing cell blockage at G0/G1 stage,which maybe resulted from the inactivation of CDK2 and Cyclin E.

Key concepts: Cell cycle, Cyclin-dependent kinase 2, Cyclin E, Resveratrol, Cell biology, Chemistry, Cell growth, Hepatoblastoma

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