The relationship of serum homocysteine with collagen IV and serum cystatin C in patient with diabetic nephropathy

Objective To investigate the relationship of serum homocysteine(Hcy) with collagen Ⅳ(ⅣC) and serum cystatin C(CysC) in patient with diabetic nephropathy(DN).MethodsOne hundred and fifty patients with type 2 diabetes mellitus were equally divided into three groups of A(normal albuminuria),B(microalbuminuria) and C(clinical proteinuria).Fifty healthy people were taken as the controls(group D).Serum levels of Hcy,ⅣC and CysC were measured using ELISA method,radioimmunoassay and particle-enhanced turbidimetric immunoassay,respectively.The relationship of serum Hcy with ⅣC and CysC were analyzed.Results Serum levels of Hcy,ⅣC and CysC in groups of A,B and C were significantly higher than those in group D(P0.01),which in groups of B and C were significantly higher than those in group A(P0.01).Serum level of Hcy was positively correlated with that of ⅣC and CysC(r1=0.58,r2=0.68,P0.05).Conclusion High level of serum Hcy results in an increase of serum ⅣC and CysC,which may play an important role in the occurrence and development of DN.