Inductive Technology for Stolon of Fragaria ananassa Duchesne(Strawberry) Seedlings in vitro

[Objective] The aim was to discuss concentration ratio of best hormone which induce stolon of strawberry in vitro,to provide basis for further detoxification of strawberry.[Method] Concentration ratio of best hormone which effect the appearance of stolon of strawberry in vitro were screened through uniform design experiments,and the effect of main factors and levels of each step were discussed to receive shoot tip of strawberry which were available to virus eradication.[Result] The results showed that the medium of LS+6-BA 1.00 mg/L+GA3 1.90 mg/L+KT 1.00 mg/L was suitable for Toyonoka strawberry stolon induction in vitro,on that medium,the frequency of induction was more than 99.5%.[Conclusion] The experiment results proved that which is possible to induced stolon of seedling in vitro from strawberry,and laying the foundation for virus eradication of strawberry with shoot tip.