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2011•Zhongguo redai yixueRequires access

Detection of IL-4,IL-2 and IFN-γ levels in supernatant of PBMCs from patients with atopic dermatitis

Baojian Chen

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Abstract

Objective To understand the levels of interleukin 4(IL-4),interferon γ(IFN-γ) and interleukin 2(IL-2) in culture supernatants of peripheral blood mononuclear cells(PBMCs) from patients with atopic dermatitis(AD).Methods The levels of IL-4、IFN-γ、IL-2 in supernatants of PBMCs from 97 patients with AD and 20 normal controls were measured by ELISA method.PBMCs were cultured for 72 hours in vitro.Results The levels of IL-4 and IL-2 were both significantly higher than those of the controls(P0.01 or 0.05)and levels of IFN-γ was significantly lower than that of in the controls.Conclusion The change of the levels of IL-4、IFN-γ、IL-2 played an important role in the pathogenesy atopic dermatitis.

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Objective To understand the levels of interleukin 4(IL-4),interferon γ(IFN-γ) and interleukin 2(IL-2) in culture supernatants of peripheral blood mononuclear cells(PBMCs) from patients with atopic dermatitis(AD).Methods The levels of IL-4、IFN-γ、IL-2 in supernatants of PBMCs from 97 patients with AD and 20 normal controls were measured by ELISA method.PBMCs were cultured for 72 hours in vitro.Results The levels of IL-4 and IL-2 were both significantly higher than those of the controls(P0.01 or 0.05)and levels of IFN-γ was significantly lower than that of in the controls.Conclusion The change of the levels of IL-4、IFN-γ、IL-2 played an important role in the pathogenesy atopic dermatitis.

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Available abstract

Objective To understand the levels of interleukin 4(IL-4),interferon γ(IFN-γ) and interleukin 2(IL-2) in culture supernatants of peripheral blood mononuclear cells(PBMCs) from patients with atopic dermatitis(AD).Methods The levels of IL-4、IFN-γ、IL-2 in supernatants of PBMCs from 97 patients with AD and 20 normal controls were measured by ELISA method.PBMCs were cultured for 72 hours in vitro.Results The levels of IL-4 and IL-2 were both significantly higher than those of the controls(P0.01 or 0.05)and levels of IFN-γ was significantly lower than that of in the controls.Conclusion The change of the levels of IL-4、IFN-γ、IL-2 played an important role in the pathogenesy atopic dermatitis.

Key concepts: Peripheral blood mononuclear cell, Atopic dermatitis, Immunology, Medicine, Interleukin, Interleukin 4, Interleukin 5, Interferon

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Detection of IL-4,IL-2 and IFN-γ levels in supernatant of PBMCs from patients with atopic dermatitis — Research Paper | ScholarLens