Expression of VEGF and SDF-1/CXCR4 in cord blood endothelial progenitor cells induced by GLP-1 gene

【Objective】 To discuss the influence of GLP1 genes' expression-regulation on VEGF and SDF-1/CXCR4 in EPCs.【Methods】 After construction of GLP-1 expression vector and transfection of EPCs,the vectors were transfected into EPCs in blank control,0.3 μg,0.5 μg and 0.8 μg concentration.The transfection effects were determined by RT-PCR.The mRNA and protein expression of VEGF,SDF-1 and CXCR4 were assayed by RT-PCR and western blotting.【Results】 In different vectors concentration,0.5 μg and 0.8 μg concentration of vectors showed better effects of transfection.RT-PCR showed that VEGF and SDF-1 had the highest mRNA expression after 0.5 μg vector transfection compared with control group(P 0.05).CXCR4 mRNA expression were the highest in 0.5 μg and 0.8 μg compared with control group(P 0.05).The result of western blotting showed that the protein expression of VEGF,SDF-1 and CXCR4 had different up-regulation after transfection.【Conclusions】 GLP-1 could induce expression of VEGF and SDF-1/CXCR4 in cord blood endothelial progenitor cells.GLP-1 could promote proliferation,differentiation,and migration of EPCs by up-regulation of VEGF and SDF-1/CXCR4.