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[Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells].

Weidong Wu, Chihua Fang, Zheng-xin Yang, Jia-jin Bao

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Abstract

OBJECTIVE: To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells. METHODS: Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR. RESULTS: After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05). CONCLUSION: Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.

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What this paper is about

OBJECTIVE: To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells. METHODS: Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR. RESULTS: After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05). CONCLUSION: Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.

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Available abstract

OBJECTIVE: To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells. METHODS: Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR. RESULTS: After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05). CONCLUSION: Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.

Key concepts: Transfection, Small interfering RNA, Cancer research, Breast cancer, Molecular biology, Cell culture, Reverse transcription polymerase chain reaction, Messenger RNA

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[Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells]. — Research Paper | ScholarLens