Multiplex PCR for the detection of the 9 enterotoxin genes in foodborne staphylococcus aureus

OBJECTIVE To establish a multiplex PCR method for detecting 9 enterotoxin genes in foodborne staphylococcus aureus, and study the distribution of 9 enterotoxin genes in foodborne staphylococcus aureus. METHODS Two multiplex PCR methods were established to detect 9 enterotoxin genes in 144 foodborne staphylococcus aureus strains , and analyzed their distribution. RESULTS The multiplex PCR method was specific, efficient; The detection rates of 9 enterotoxin genes in 144 foodborne staphylococcus aureus strains were in descending order for SEU ( 25.69% ) , SEG ( 22.22% ) , SEM ( 20.83% ) SEK ( 20.14% ) , SEQ ( 18.06% ) , SEH ( 15.97% ) , SEN ( 10.42% ) , SEJ ( 8.33% ) , SEL ( 5.56% ) ; 52.78% of the strains contained at least one gene of these 9 enterotoxin genes, 34.03% of the strains contained two or more genes of these 9 enterotoxin genes. CONCLUSION The multiplex PCR method is highly specific, simple and convenient; It can be used for the study of the enterotoxin genes distribution in staphylococcus aureus; All 9 enterotoxin genes have been detected in 144 foodborne staphylococcus aureus strains, in which the detection rates of SEU, SEG, SEM, SEK are higher.