Isolation of a phenanthrene-degrading bacteria and phenanthrene biodegradation kinetics

A phenanthrene degrading bacteria,named y-8,was screened from the thick oil polluted soil of Karamay Oilfield.The strain y-8 could grow with phenanthrene as sole source of carbon;it was identified as Pseudomonas geniculata according to its morphological,physiological,biochemical and 16S rDNA sequence characteristics.Inoculate 2% of y-8 to phenanthrene solution of 100 mg/L,93.7% of phenanthrene was degraded in 72 h under the culture conditions of 30 ℃,pH 7.0 and shaking speed 140 r/min.The strain exhibited good tolerance to phenanthrene(3 000 mg/L).The kinetics of phenanthrene biodegradation was analyzed,base on which,the exponential model was established.The biodegradation process could be well fitted by first-order kinetics,and the half value period was 91.01 h.