Effects of venous hypertension on the expression of soluble adhesion molecules in local plasma of the lower extremities

Objective To investigate the effects of venous hypertension on the expression of soluble adhesion molecules in local plasma of the lower extremities.Methods Twenty patients with chronic venous insufficiency(CVI)were selected.Venous insufficiency only in one side was verified by Duplex ultrasonic scanning and venography.After a stasis of 30 min,blood was sampled both in the varicose vein(group Vh)and in the normal vein of lower limbs(group Nh).At the same time,the venous blood was sampled in the brachial vein as a group of control.After relieving stasis(supine position) for 15min,venous blood was sampled in the varicose vein(group Vr)and normal vein(group Nr).sICAM-1 and sVCAM-1 were measured in plasma with use of ELISA kits.Results(1)After blood stasis,plasma sICAM-1 and sVCAM-1 were significantly increased in group Vh and group Nh as compared with control group(P0.01),but there was no significant difference between group Vh and group Nh(P0.05);(2)After a relief of stasis for 15 min,the sICAM-1 and sVCAM-1 were decreased in the normal vein of lower limb(P0.05);(3)In the varicose vein,there was no variation in the sICAM-1 and sVCAM-1 after relieving stasis for 15min.Conclusion In the conditions of blood stasis and hypoxia,no matter in varicose veins or normal veins,there are local leukocytes adhension and vascular endothelial cells injury.In a short time after relieving blood stasis and hypoxia,the local adhesion molecules remain high-level in varicose veins,suggesting the inflammatory response persisted.On the contrary,local adhesion molecules were descended quickly in normal veins.Probably the continuity of local inflammatory response plays an important role in the developments of CVI.