Impact of in situ gel OALA on biological activity of DDP

【Objective】 To observe the impact of in situ gel OALA on biological activity of chemotherapeutic drug DDP,and provide the experimental basis for OALA to load DDP.【Methods】 ①The cytotoxicity of OALA in vitro: SRB method was used to evaluate the effects of OALA in different concentrations on the proliferation of tumor cells(A549 lung cancer cells,MCF-7 breast cancer cells,LOVO colon cells),then determine the non-toxic concentration of OALA in these cells in vitro.②The impact of OALA on the toxicity of DDP in vitro: Firstly pre-test to determine the IC50 of chemotherapeutic drug DDP action on A549,MCF-7,LOVO and L929 cell lines respectively,then MTT method was used to evaluate the impact of OALA on cytotoxicity of DDP in vitro.③The impact of OALA on the toxicity of DDP in vivo: Firstly pre-test to determine the lethal dose of chemotherapeutic drugs DDP action on KM mice,then to intervene under different lethal dose injecting OALA intravenously,to compare mortality with the group of single DDP administrating intraperitoneally.④The impact of OALA on the in vivo antitumor activity of DDP: The EAC tumor-bearing mice were used as the experimental model to observe the impact of different doses of DDP and DDP combined with OALA on tumor growth in mice,then tumor growth inhibition rate was taken as its index.【Results】 ①The results of cytotoxicity in vitro showed that OALA-30 μg/mL had no influence on the proliferation of A549,MCF-7 and LOVO cells.②OALA-30 μg/mL was non-toxic or the inhibition was weak for A549,MCF-7,LOVO and L929 cell lines,OALA combined with three chemotherapeutic drugs would not increase or decrease the cytotoxicity of drug itself.③OALA could increase the animal mortality in DDP low dose group,but had no significant effect on the animal mortality of DDP high and median.④OALA had no effect on the anti-tumor activity of DDP.【Conclusion】 In vitro,OALA had no effect on the cytotoxicity of DDP;In vivo,OALA could differently interfere with the toxicity of DDP,but had no influence on its anti-tumor activity.