Effects of atorvastatin pretreatment on expressions of HIF-αand VEGF at different time points in myocardium tissue of rats after myocardial ischemia reperfusion

Objective To explore the effects of atorvastatin pretreatment(AT)on the expressions of HIF-αand VEGF protein and mRNA at different time points in myocardium tissue of the rats after myocardium ischemia reperfusion(I/R)and to elucidate the protective effect of AT on myocardium tissue of the rats.Methods 50healthy male Wistar rats were randomly divided into sham operation group,ischemia 30 min reperfusion 2hmodel(I30min R2h)group,ischemia 30min reperfusion 6hmodel(I30min R6h)group,ischemia 30min reperfusion 2hatorvastatin(I30min R2h AT,10mg·kg-1)group,and ischemia 30min reperfusion 6hatorvastatin(I30min R6h AT,10mg·kg-1)group;there were 10rats in each group.I/R model was prepared by ligating the left anterior descending coronary artery(LAD)method.The ratios of mypcordial infarction area of the rats in various groups were detected,the expressions of HIF-αand VEGF protein were detected with immunohistochemical method in myocardium tissue of the rats,the expression levels of HIF-αand VEGF mRNA in myocardium tissue of the rats were detected by RT-PCR method.Results Compared with sham operation group,the ratios of myocardial infarction area of the rats in I30min R2h group and I30minR6hgroup were increased(P0.01);the ratios of myocardial infarction area of the rats in I30min R2h AT group and I30min R6hgroup were lower than those in I30minR2hgroup and I30min R6h group(P0.01).Compared with sham operation group,the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA in I30min R2h group,I30min R6h group,I30min R2h AT group,and I30min R6h AT group were significantly increased(P0.01);the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA of the rats in I30min R6h group were lower than those in I30minR2hgroup,and the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA of the rats in I30min R6h AT group were lower than those in I30min R2h AT group(P0.01).Compared with I30min R2h group,the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA of the rats in I30min R2h AT group were significantly increased(P0.01);compared with I30min R6h group,the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA of the rats in I30min R6h AT group were significantly increased(P0.01).Conclusion I/R injury can early cause up-regulation of the levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA in rats,and AT can significantly up-regulate the expression levels of HIF-αand VEGF protein and HIF-αand VEGF mRNA,which suggests that AT has the protective effect on the myocardium tissue of rats.