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2015•Guangdong nongye kexueOpen access

Comparative analysis of genetic diversity revealed by genomic-SSR and EST-SSR markers in interspecies of Stylosanthes

Ding Xi-pen

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Abstract

To explore the genetic differences between genomic-SSR and EST-SSR in different species of Stylosanthes, the genetic diversity of 12 accessions from different Stylosanthes species was analyzed by two types of SSR markers. Results showed that, compared with genomic-SSR, EST-SSR markers were superior in versatility for different species of Stylosanthes. The average of number of alleles and the polymorphic information content(PIC)value detected by EST-SSR was 4.6 and 0.610, respectively. The average of number of alleles and PIC value detected by genomic-SSR was 5.6 and 0.702, respectively. Cluster analysis indicated that two SSR markers could effectively identify all Stylosanthes accessions.

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What this paper is about

To explore the genetic differences between genomic-SSR and EST-SSR in different species of Stylosanthes, the genetic diversity of 12 accessions from different Stylosanthes species was analyzed by two types of SSR markers. Results showed that, compared with genomic-SSR, EST-SSR markers were superior in versatility for different species of Stylosanthes. The average of number of alleles and the polymorphic information content(PIC)value detected by EST-SSR was 4.6 and 0.610, respectively. The average of number of alleles and PIC value detected by genomic-SSR was 5.6 and 0.702, respectively. Cluster analysis indicated that two SSR markers could effectively identify all Stylosanthes accessions.

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Available abstract

To explore the genetic differences between genomic-SSR and EST-SSR in different species of Stylosanthes, the genetic diversity of 12 accessions from different Stylosanthes species was analyzed by two types of SSR markers. Results showed that, compared with genomic-SSR, EST-SSR markers were superior in versatility for different species of Stylosanthes. The average of number of alleles and the polymorphic information content(PIC)value detected by EST-SSR was 4.6 and 0.610, respectively. The average of number of alleles and PIC value detected by genomic-SSR was 5.6 and 0.702, respectively. Cluster analysis indicated that two SSR markers could effectively identify all Stylosanthes accessions.

Key concepts: Stylosanthes, Biology, Genetic diversity, Microsatellite, Allele, Genetic marker, Genotype, genomic DNA

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Comparative analysis of genetic diversity revealed by genomic-SSR and EST-SSR markers in interspecies of Stylosanthes — Research Paper | ScholarLens