Study on the antibiotic resistance and serotyping of quinolone resistance of Pseudomonas aeruginosa

Objectives To explore the antibiotic resistance and reducing effect on quinolone resistance of Pseudomonas aeruginosa using pump inhibitor,and to study the serotyping in Guangzhou.Methods 127 strains P.aeruginosa were identified and the antibiotic sensitivity test were performed by VITEK-2 system.Using the method of agar dilution to measure the Minimum inhibitory concentrations(MIC) of the quinolone-resistant isolates,along with carbonyl cyanide-3-chloro-phenylhydrazone(CCCP) and ciprofloxacin.And those isolates were typed with IATS glass agglutination.Results The sensitivity rate of quinolone-resistant strains were piperacillin/tazobactam(65.5%),amikacin(64.4%),piperacillin(51.7%),tobramycinfour(50.6%).And the sensitivity rate of quinolone-sensitive strains were meropenem(97.5%),levofloxacin(97.5%),tobramycinfour(95.0%).To 13 kinds of antibiotics,the rates of resistance isolates were apparently lower than the sensitive isolates(P0.001).The pump inhibitor CCCP could reduce MIC.Typing rate was 93.1%,serotype of resistance isolates were type B(20.7%),type L(19.5%).Conclusions To all antibiotics the sensitivity rate of quinolone-resistant P.aeruginosa were decreased,and had multidrug resistance to antibiotics tested.By using antibiotic with pump inhibitor,the sensitivity rate can be increased.Serotyping can be used to analyse the hospital epidemic of P.aeruginosa.