Genetic analysis of Rongchang pig and two breeds of miniature pigs by microsatellite markers

Rongchang pig is a superior Chinese local breed,and both Bama Xiang pig and Guizhou Xiang pig are special Chinese miniature pigs,distinctive genetic resources in China.Nineteen microsatellite loci recommended by PigMap diversity studies were screened for Rongchang pig,line B of Rongchang pig,Bama miniature pig,Guizhou miniature pig and Largewhite pig.All loci were polymorphic and 10-23 alleles were detected in these 5 breeds.The most genetic variations were detected in Line B of Rongchang pigs,followed by Rongchang pigs.Less genetic variations were detected in 2 miniature pig breeds.In addition,more alleles were detected in Bama miniature pigs(121) than in Guizhou miniature pigs(114),but the mean expected heterozygosities were contrary,which suggest lower genetic heterozygosity and less genetic diversities in Bama miniature pigs.The least genetic variations were detected in Largewhite pigs.From shared alleles,Rongchang pig(30.22%) accounts more than Largewhite pig(24.37%) for the genetic origin of Line B of Rongchang pig;less alleles were shared by Largewhite pig and other pig breeds(21.24%-25.12%);and the most alleles were shared by Bama miniature pigs and Guizhou miniature pigs.Genetic differentiation of genetic distance suggested by Fst and Nei were comparable.Phylogenetic tree derived from neighbor-joining was somewhat different with that derived from the maximum likelihood methods.