2014•Journal of Dalian Ocean UniversityRequires access

The expression of Mn-SOD gene in larval mantis shrimp Oratosquilla oratoria under salinity stress

Haiyin Liu

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Abstract

The expression of Mn-SOD gene was detected in pseudozoea( Z9-Z11) of mantis shrimp Oratosquilla oratoria when the pseudozoea was exposed to a salinity of 12. 8,16. 3,19. 8,23. 3,26. 8( control group),30. 3, 33. 8,and 37. 3 for 1. 5,3,6,9,12,24,36,48,and 72 h by real time PCR. The results showed that within the different salinity exposure of 6,9,12,48,and 72 h there was very significantlys and significantly lower Mn-SOD gene expression levels than that in the control group( P0. 01 or P0. 05) and there was mostly higher Mn-SOD gene expression levels in salinity of 23. 3,and 30. 3 than that in the 37. 3 or 12. 8 groups( P0. 01). In the salinity exposure from 24 h to 36 h,there was significantly higher Mn-SOD gene expression levels than that in the control group except salinity of 12. 8,and 16. 3 than that in the control group( P0. 01). The gene expressions in different salinity stress were found to be decrease firstly,then increased and finally decreased gradually during 72 hours except the maximal and minimal salinity. At a salinity of 12. 8,16. 3,19. 8,23. 3,30. 3,33. 8,and 37. 3, the maximum gene expression was observed in 1. 5,12,24,24,24,24,and 24 h salinity exposure,respectively. The mantis shrimp larvae had a salinity adaptation trend from unadaptation,via the most unadaptation,and gradual adaptation,to unadaptation.

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The expression of Mn-SOD gene was detected in pseudozoea( Z9-Z11) of mantis shrimp Oratosquilla oratoria when the pseudozoea was exposed to a salinity of 12. 8,16. 3,19. 8,23. 3,26. 8( control group),30. 3, 33. 8,and 37. 3 for 1. 5,3,6,9,12,24,36,48,and 72 h by real time PCR. The results showed that within the different salinity exposure of 6,9,12,48,and 72 h there was very significantlys and significantly lower Mn-SOD gene expression levels than that in the control group( P0. 01 or P0. 05) and there was mostly higher Mn-SOD gene expression levels in salinity of 23. 3,and 30. 3 than that in the 37. 3 or 12. 8 groups( P0. 01). In the salinity exposure from 24 h to 36 h,there was significantly higher Mn-SOD gene expression levels than that in the control group except salinity of 12. 8,and 16. 3 than that in the control group( P0. 01). The gene expressions in different salinity stress were found to be decrease firstly,then increased and finally decreased gradually during 72 hours except the maximal and minimal salinity. At a salinity of 12. 8,16. 3,19. 8,23. 3,30. 3,33. 8,and 37. 3, the maximum gene expression was observed in 1. 5,12,24,24,24,24,and 24 h salinity exposure,respectively. The mantis shrimp larvae had a salinity adaptation trend from unadaptation,via the most unadaptation,and gradual adaptation,to unadaptation.

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Available abstract

The expression of Mn-SOD gene was detected in pseudozoea( Z9-Z11) of mantis shrimp Oratosquilla oratoria when the pseudozoea was exposed to a salinity of 12. 8,16. 3,19. 8,23. 3,26. 8( control group),30. 3, 33. 8,and 37. 3 for 1. 5,3,6,9,12,24,36,48,and 72 h by real time PCR. The results showed that within the different salinity exposure of 6,9,12,48,and 72 h there was very significantlys and significantly lower Mn-SOD gene expression levels than that in the control group( P0. 01 or P0. 05) and there was mostly higher Mn-SOD gene expression levels in salinity of 23. 3,and 30. 3 than that in the 37. 3 or 12. 8 groups( P0. 01). In the salinity exposure from 24 h to 36 h,there was significantly higher Mn-SOD gene expression levels than that in the control group except salinity of 12. 8,and 16. 3 than that in the control group( P0. 01). The gene expressions in different salinity stress were found to be decrease firstly,then increased and finally decreased gradually during 72 hours except the maximal and minimal salinity. At a salinity of 12. 8,16. 3,19. 8,23. 3,30. 3,33. 8,and 37. 3, the maximum gene expression was observed in 1. 5,12,24,24,24,24,and 24 h salinity exposure,respectively. The mantis shrimp larvae had a salinity adaptation trend from unadaptation,via the most unadaptation,and gradual adaptation,to unadaptation.

Key concepts: Salinity, Gene expression, Shrimp, Gene, Biology, Animal science, Genetics, Fishery

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