Study on Factors Influencing Efficiency of Sweetpotato Transformation

Objective: Sweetpotato transformation efficiency would been raised.Methods: In this paper,the stem explants of many superior cultivars of sweetpotato and LBA4404/pSP10Z strain of Agrobacterium tumefaciens were used as test material.The effect of many factors on sweetpotato genetic transformation had been studied.Results: Inoculation duration had effect on efficiency of transformation.The suitable time for inoculation duration was 2～15min.The highest frequency of transformation was 32.14%.The transformed receptors of different genotypes had different sensitivity to the same Agrobacterium strain.Transformation efficiency had been raised 2.02 times when 50μmol/L acetosyringone was presence in culture of bacterium at the last 30min.Transformation efficiency had been raised 6.11 times when 50μmol/L acetosyringone was presence in co-culture medium.The pH 4.8 of co-culture medium could raise transformation efficiency too.The transformation efficiency had been raised 1.73 times.Conclusion: This paper supplied some gist for the establishment of fast and efficient genetic transformation system of sweetpotato mediated by A.tumefacienens.