The research of survivin gene antisense oligonucleotide enhances the sensitivity of gastric cancer cells to hydroxycamptothecine.

Objective To investigate the effects of survivin antisense oligonucleotide(ASODN)on gastric cell line BGC-823.Methods Lipofectin was used to encapsulate survivin ASODN an sense oligonucleotides(SODN) in trans-fection.Viability of BGC-823 cells was assessed by methyl thiazolyl tetrazolium(MTT) method,then the apoptotic cells was detected by flow cytometry.The ultrastructural changges of cancer cells were observed under trans-mission electron microscope.The expression of survivin mRNA was detected by reverse transcription-Polymerase chain reaction(RT-PCR).Results Survivin ASODN could inhibite proliferative activity and induced apoptosis of gastric cancer cells in a dose-dependent manner.The ultrastructural change of the BGC-823 cells treated with hydroxycamptothecine(OPT) and ASODN were as following:the nucleolar marginnation was frequent in nucleus.The expression of survivin in BGC-823 cells treated by OPT was higher than the control and ASODN+OPT groups.Conclusion Survivin ASODN can inhibit proliferation and induce apoptosis by inhibiting the expression of survivin in BGC-823 cells.Survivin ASODN could enhance sensitivity of BGC-823 cells to OPT,even they accepted several times treation by OPT.