GCIP-27 inhibits the right ventricle remodeling in monocrotaline-treated rats

Aim The effect and mechanism of GCIP-27 on the right ventricle remodeling by monocrotaline (MCT) were explored.Methods Sprague-Dawley rats were randomly divided into the normal group,MCT group and the low and high dose GCIP-27 treatment group.Pulmonary arterial systolic pressure (PASP),pulmonary artery diastolic pressure (PADP) and mean pulmonary arterial pressure (MPAP) were measured by right cardiac catheter on the 22 th day.RV and left ventricle plus septum were weighed separately.Right ventricular hypertrophy index (RVHI) was measured by the weight ratio of RV to left ventricle plus septum.The histological examination of right ventricle were executed by light microscope and transmission electron microscope.Immunohistochemical analysis was performed on the paraffin-embedded right ventricle tissue using the anti-PCNA primary antibodies.Results GCIP-27 (90 μg·kg-1) significantly inhibited the progression of PASP and MPAP and GCIP-27 (30 μg·kg-1) only reduced the progression of PASP.GCIP-27 significantly inhibited the increase of RVHI and cardiomyocytes hypertrophy versus MCT group.The swollen and misshapen mitochondria could be improved by GCIP-27.And the PCNA expression caused by MCT could be inhibited by GCIP-27 in right ventricle cardiomyocytes.Conclusion GCIP-27 can attenuate the right ventricular remodeling siginificantly in monocrotaline-treated rats.