The Relationship Between Expression of Survivin mRNA and Protein with Apoptosis in Colorectal Carcinoma

Objective To study the significances of survivin mRNA and protein expression in colorectal carcinomas and the relationship between them with apoptosis index (AI).Methods The copies of survivin mRNA were measured by quantitative PCR with a molecular beacon probe. The expression of protein was investigated by immunohistochemistry SP method. AI was analyzed with TUNEL.Results The copies of survivin mRNA and the positive rate of protein in colorectal carcinoma were significantly higher than those in normal tissues. In contrast, AI in colorectal carcinoma was significantly lower than those in normal tissues (P0.05). The absolute copies of survivin mRNA were related with lymph node metastases and different histological types. The positive rate of protein expression was related with tumor locus and survival. AI in negative cases of protein expression was significantly higher than in positive cases (P0.05). There were not correlations between the copies of survivin mRNA and protein expression (P0.05).Conclusion The copies of survivin mRNA and the positive rates of protein expression were higher in colorectal carcinoma. It may be a potential molecular indication to predict the prognosis.