ScholarLens Back to search
2001•Zhonghua shenzangbing zazhiRequires access

Effects of interleukin-13 on cell proliferation and interleukin-1β production in mesangial cells

Liming Jiang

Open publisher page 0 citations

Abstract

Objective To explore the effects of interleukin-13(IL-13) on cell proliferation and interleukin-113(IL-1β) production in mesangial cell. Methods Cell proliferation was tested by MTT method. The expression of IL-1β mRNA in mesangial cells was evaluated by RT-PCR. The protein synthesis of IL-1β in mesangial cells was measured by ELISA. Results IL-13. in the range of concentration from 1 ng/ml to 100 ng/ml. could inhibit the proliferation of mesangial cells with or without inducing by LPS: Il-13. in the same range of concentration, also inhibited obviously the expression of IL-1β mRNA and the protein release of IL-1β induced by LPS in mesangial cells. IL-13 in 10 ng/ml could inhibit almost completely the expression of IL-1β mRNA induced by LBS. Conclusion IL-13 can inhibit the proliferation of mesangial cells and IL-1βproduction induced by LBS in mesangial cells.

About this research paper

What this paper is about

Objective To explore the effects of interleukin-13(IL-13) on cell proliferation and interleukin-113(IL-1β) production in mesangial cell. Methods Cell proliferation was tested by MTT method. The expression of IL-1β mRNA in mesangial cells was evaluated by RT-PCR. The protein synthesis of IL-1β in mesangial cells was measured by ELISA. Results IL-13. in the range of concentration from 1 ng/ml to 100 ng/ml. could inhibit the proliferation of mesangial cells with or without inducing by LPS: Il-13. in the same range of concentration, also inhibited obviously the expression of IL-1β mRNA and the protein release of IL-1β induced by LPS in mesangial cells. IL-13 in 10 ng/ml could inhibit almost completely the expression of IL-1β mRNA induced by LBS. Conclusion IL-13 can inhibit the proliferation of mesangial cells and IL-1βproduction induced by LBS in mesangial cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the effects of interleukin-13(IL-13) on cell proliferation and interleukin-113(IL-1β) production in mesangial cell. Methods Cell proliferation was tested by MTT method. The expression of IL-1β mRNA in mesangial cells was evaluated by RT-PCR. The protein synthesis of IL-1β in mesangial cells was measured by ELISA. Results IL-13. in the range of concentration from 1 ng/ml to 100 ng/ml. could inhibit the proliferation of mesangial cells with or without inducing by LPS: Il-13. in the same range of concentration, also inhibited obviously the expression of IL-1β mRNA and the protein release of IL-1β induced by LPS in mesangial cells. IL-13 in 10 ng/ml could inhibit almost completely the expression of IL-1β mRNA induced by LBS. Conclusion IL-13 can inhibit the proliferation of mesangial cells and IL-1βproduction induced by LBS in mesangial cells.

Key concepts: Mesangial cell, Cell growth, Interleukin, Chemistry, Molecular biology, Messenger RNA, Cell, MTT assay

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of interleukin-13 on cell proliferation and interleukin-1β production in mesangial cells — Research Paper | ScholarLens