Construction of Suppression Subtractive cDNA Library and Screening and Preliminary Identification of Differentially Expressed Genes in the Ovary of the Different Egg Production Performance in Chicken

In order to screen the chief genes and regulatory factors relevant to egg performance in chicken,suppression subtractive hybridization(SSH) was employed to construct the SSH cDNA library in the ovary from commercial Hyland brown layers of high egg production and Dagu layers of low egg production at 20 weeks of age in the present study.The inserted fragment sizes focused around 500 bp and 720 clones were verified by PCR,in which 657 clones were used to perform dot blotting hybridization.A total of 536 clones were obtained and 100 clones were randomly picked and sequenced.Fifty two differentially expressed genes were isolated by homologous BLAST search.Besides 3 unknown function genes,the others implicated in cell propagation and regulation,folliculogenesis,and nutritional metablic pathway of calcium,phosphorus and protein,and so on.Four differentially expressed genes probably impacting egg production of chicken were identified by using semi-quantitative RT-PCR.