A murine model of obliterative bronchiolitis after lung transplantation

Objective To establish a murine model of obliterative bronchiolitis(OB) after lung transplantation.Methods Pathogen-free male C57BL /6(n = 25 ) and BALB / c(n = 5 ) mice weighing 20 ~25 g were used for experiments.Weight-matched mice were assigned to two groups for orthotopic tracheal transplant.Experimental group:the trachea from BALB / c(n = 5 ) was transplanted to C57BL /6(n = 10);Control group:the trachea from C57BL /6(n = 5) was transplanted to C57BL /6(n = 10) mice.Native and transplanted lungs were harvested,stained with HE,and examined under light microscopy.Results The mortality was 0%(0/20) during the process of model establishment and four-week mortaUty was 5%(1/20).Pathology revealed the trachea endothelial cells in experimental group were damaged,accompanied by enhanced inflammatory cell infiltration and fibro hyperplasia.Incidence of obliterative bronchiolitis in mice of experimental group was approximately 100%,but control group showed no pathological changes in lung tissue. Conclusion Murine trachael transplantation model has been established successfully may be used to study the mechanism of OB.