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Application of Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) Monolith in Plasmid Analysis

Minlian Zhang

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Abstract

Anion-exchange poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) (PGDT) monolith with superporous pores with the diameters range of 10～300 μm was synthesized, which was used to analyze plasmid standard and cell lysate. The analysis results were compared with commercial columns, including TSK DNA–NPR and CIM DEAE Disk Monolithic Column. The prepared PGDT monolith could effectively absorb plasmid and separate it from impurities, with its results similar to TSK and CIM

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What this paper is about

Anion-exchange poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) (PGDT) monolith with superporous pores with the diameters range of 10～300 μm was synthesized, which was used to analyze plasmid standard and cell lysate. The analysis results were compared with commercial columns, including TSK DNA–NPR and CIM DEAE Disk Monolithic Column. The prepared PGDT monolith could effectively absorb plasmid and separate it from impurities, with its results similar to TSK and CIM

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Available abstract

Anion-exchange poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) (PGDT) monolith with superporous pores with the diameters range of 10～300 μm was synthesized, which was used to analyze plasmid standard and cell lysate. The analysis results were compared with commercial columns, including TSK DNA–NPR and CIM DEAE Disk Monolithic Column. The prepared PGDT monolith could effectively absorb plasmid and separate it from impurities, with its results similar to TSK and CIM

Key concepts: Monolith, Glycidyl methacrylate, Divinylbenzene, Monolithic HPLC column, Methacrylate, Plasmid, Lysis, Chemistry

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Application of Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) Monolith in Plasmid Analysis — Research Paper | ScholarLens