2006•Biotechnology(Faisalabad)Requires access
Construction of the Transgene Human c-myc Cell
LI Hou-da
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Abstract
Objectives:To construct human c-myc transgene cell.Methods: Human c-myc cDNA was recombinated into retroviral vector plasmid pGEZ-Term.The recombinant of pGEZ-Term/c-myc and HIT45 and HIT60 were then transduced into the packaging cell line(293T) by means of lipofectin.Recombinant viruses were obtained.The retroviral titer was detected by infecting NIH3T3 cell with supernatants of 293T cell.The recombinant viruses infected L929 cell.By using the medium containing Zeocin for clone selection.Results: c-myc transgene cells were constructed.In addition,the supernatants of the L929 cells transduced with retroviral vector containing c-myc cDNA could significantly promote the proliferation of L929 cells.Conclusion:Transgene cells were obtained by retroviral transfer.