THE EFFECTS OF ICAM 1 AND VCAM 1 ON THE ADHERENCE OF ACTIVATED LYMPHOCYTES TO HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

Objective:To explore the effects of ICAM 1 and VCAM 1 on the adherence of rIL 2 activated umbilical lymphocytes to human umbilical vein endothelial cells(HUVEC). Methods:① Activated umbilical lymphocytes were put on the surface of the cultured HUVEC sheet.After a certain period of time, the adherence rate was calculated. ② The phenotypes of adherent lymphocytes were studied with APAAP method. Results:①ICAM 1 and VCAM 1 McAb obviously lower the adherent activity of activated lymphocytes.The former decreased the adherent rate from 56.54% to 41.6%(P0.05),and the latter to 30.3%(P0.05). ② ICAM 1 McAb lowered the adherence of CD8 +,raised the adherence of CD4 +,and have no significant effect on CD56 +,while VCAM 1 McAb increased the adherence of CD8 +,lowered the adherence of CD56 + and have no significant effect on CD4 +. Conclusion:The adherent activity of activated lymphocytes is stronger obviously than that of unactivated ones.ICAM 1 and VCAM 1 may participate in the regulation of the adherence of activated lymphocytes to HUVEC.