Effect of interference of MTA1 by shRNA on the biological behaviors and expression of related proteins in breast cancer MDA-MB-231 cells

Objective:To silence the expression of metastasis-associated gene 1(MTA1) in breast cancer cell line MDA-MB-231 by using short hairpin small interfering RNA(shRNA) and observe its effects on expression of 15-lipoxygenase 2(15-LOX-2),p53 and bcl-2 proteins.Methods:The shRNA-MTA1 plasmid was stably transfected into MDA-MB-231 cells and the cell proliferation was evaluated using MTT assay.The cell cycle distribution and apoptosis were analyzed using flow cytometry.The mRNA and protein expression levels of MTAl,15-LOX-2,p53 and bcl-2 were determined using RT-PCR and Western blotting,respectively.Results:shRNA-MTAl significantly suppressed the expression of MTA1 gene in MDA-MB-231 cells,inhibited the proliferation,induced apoptosis,and arrested the cells in G1 phase.The difference was significant compared with control group(P0.01).Expression levels of 15-LOX-2 and p53 were significantly up-regulated but MTAl and bcl-2 were significantly down-regulated in MDA-MB-231 cells in shRNA-MTA1 group compared with the blank control group and negative control group(P0.01).Conclusion:Silencing MTA1 gene inhibited the proliferation and induced the apoptosis of MDA-MB-231 cells.This effect may be related with up-regulation of the expression of 15-LOX-2 and p53 and down-regulation of bcl-2.