Reaction of the protected amino acid linked to the hydroxy resin

The reaction of twenty kinds of Fmoc-AA-OH linked to Wang resin using methods of acyl chloride or active ester was studied in solid-phase peptide synthesis.Expect for arginine,the yield of other amino acid linked to Wang resin was over 80%.The method of acyl chloride was better than the method of active ester for its high efficiency and convenient operation.For arginine,the yield was improved to nearly 80% by using weakly polar solvent such as DCM and simultaneously increasing the reaction time to 20 h in the method of acyl chloride.