Study on method of oxytocin induced in vitrodysmenorrhea model in mouse

Aim To establish and optimize the dysmenorrhea model of oxytocin induced in vitro uterine contraction in mice. Methods Several kinds of estrogen used to enhance the sensibility of mouse uterus were compared, the uterus was separated from the body, 0.5~10 U·L-1 oxytocin was used to induce uterine contraction, the variations after changing treatment were observed, and four parameters used to described the uterine contraction were compared. Results Oxytocin in the concentration of 5 U·L-1 was the suitable dose; the contractions were constant 5~30 min after oxytocin was given; the sensibility of uterus showed a little decrease but was not significant when body weight increased; the contractions were stable 5~30 min after oxytocin was given; the activity of uterus was completely suppressed by 1 mg·L-1 verapamil, 100 μg·L-1 nifedipine and 250 μg·L-1 chloropromazine. Conclusion Oxytocin induced in vitromouse uterine contraction model was a convenient, quick and stable in vitro dysmenorrhea model, suitable for screening dysmenorrhea therapy drugs.